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Online ISSN 1827-174X
Trombelli L. 1, Penolazzi L. 2, Torreggiani E. 2, Farina R. 1, Lambertini E. 2, Vecchiatini R. 2, Piva R. 2
1 Medico-Surgical Disciplines of Communication and Behaviour Department, Research Centre for the Study of Periodontal Diseases
University of Ferrara, Ferrara, Italy;
2 Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy
The present study evaluated human primary osteoblasts and two different osteoblast - like cell lines behaviour when cultured in presence of different hydroxyapatite-based (HA) biomaterials (SINTlife™ - FIN-CERAMICA S.p.a., Faenza, Italy; Bio-Oss™, Geistlich Biomaterials, Woulhusen, Switzerland; Biostite™ - GABA Vebas, San Giuliano Milanese, MI, Italy), focusing attention on the effect of HA/Biostite™ in terms of modulation of osteoblastic differentiation. Analysis were about adhesion, proliferation and mineralization activity. Runt-related transcription factor 2 (Runx2), Estrogen Receptor alpha (ERa) expression and alkaline phosphatase activity (ALP) were measured as osteoblastic differentiation markers. Determi-nation of viable cells was done with MTT colorimetric assay. Scanning electron microscopy (SEM) analysis was performed on biomaterial-treated cells. All hydroxyapatite-based biomaterials didn’t affect cells morphology and viability, whereas only presence of HA/Biostite™ improved cells adhesion, growth and differentiation. Adhesion and spreading of the primary cells on HA/Biostite™ were the same showed by two different osteoblast – like cell lines. These results have important implications for both tissue-engineered bone grafts and enhancement of HA implants performance, to develop new teeth’s supporting structure therapies and replacement.
language: English, Italian