Home > Journals > Minerva Stomatologica > Past Issues > Minerva Stomatologica 2000 November-December;49(11-12) > Minerva Stomatologica 2000 November-December;49(11-12):511-20

CURRENT ISSUE
 

ARTICLE TOOLS

Reprints

MINERVA STOMATOLOGICA

A Journal on Dentistry and Maxillofacial Surgery


Official Journal of the Italian Society of Odontostomatology and Maxillofacial Surgery
Indexed/Abstracted in: CAB, EMBASE, Index to Dental Literature, PubMed/MEDLINE, Scopus, Emerging Sources Citation Index


eTOC

 

ORIGINAL ARTICLES  


Minerva Stomatologica 2000 November-December;49(11-12):511-20

language: Italian

Bio-metabolic effects of two commercial fluoride-containing compounds on human keratinocytes

Mattioli-Belmonte M., Sampalmieri F., Gabbanelli F., Principato G., Biagini G., Dolci G.


PDF  


Background. Although fluoride has been used for decades either systemically or topically to prevent dental caries, the cellular and molecular mechanisms underlying its action are poorly understood.
Methods. An in vitro study of the human keratinocyte cell line NCTC 2544 was conducted in the presence of two different fluoride-containing commercial compounds (Zymafluor® and Elmex®) to investigate their toxicity threshold and the sequence of events involved in fluoride ion toxicity in this cell population.
The toxicity threshold was determined by incubating cells with rising concentrations of Zymafluor® and Elmex® for 20h. The study of the sequence of events involved in ion toxicity was performed through a time-effect study by exposing cells to 4mM fluoride ions and testing them at 2, 6, and 20h. Cell viability and ultrastructural parameters were assessed: degree of confluence, semiquantitative assessment of dead cells and debris in the supernatant, and morphology.
Results. Ultrastructural morphological analysis showed different cell behaviours with the two compounds; moreover, their toxic effect appeared to be both concentration- and time-dependent.
Conclusions. These data underline the susceptibility of the intracellular communication system to fluoride and show that exceeding the therapeutic dose of fluoride involves substantial risk of toxicity.

top of page

Publication History

Cite this article as

Corresponding author e-mail