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A Journal on Dentistry and Maxillofacial Surgery
Minerva Stomatologica 1999 November;48(11):495-500
Alteration in alfa2 integrin immunocytochemical expression on cultured human gingival fibroblasts following nicotine esposure
Leonardi R., Lanteri E., Stivala F., Caltabiano M., Senga C., Travali S.
Background. Clinical epidemiologic studies carried out in smokers versus non smokers support the concept that tobacco-related factor may affect the periodontal tissues and wound healing.
Methods. In this study, the role of nicotine on the integrin alfa2 chain immunocytochemical expression, in human gingival fibroblasts (HGF) was investigated in vitro. The kinetic induction of this type of integrin on HGF in response to increasing percentage of nicotine was been characterized. A human gingival fibroblast strain, derived from a healthy individual with non-inflamed gingiva, was used in this study. The cells were then grown on acetylated microcope slides and fixed with cold ethanol, samples were then incubated for 16 to 19 hrs at 4°C to anti-human alfa2 integrin chain monoclonal antibody.
Results. In control cultures and in HGF treated with 0.025 µM nicotine the initial higher expression of alfa2 chain decreased (grade 1 in both culture) while in HGF treated with 0.075 µM increased (grade 3). After 48 hours HGF exposed to 0.075 µM nicotine, increased further their expression of alfa2 chain.
Conclusions. The results obtained demonstrate a dose-time dependent nicotine influence on immunocytochemical expression of alfa2 integrin chain. These data suggest that nicotine may promote a collagene breakdown via an increase of alfa2 integrin chain expression.