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A Journal on Pediatrics, Neonatology, Adolescent Medicine,
Child and Adolescent Psychiatry
Indexed/Abstracted in: CAB, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,532
Minerva Pediatrica 2016 Jun 07
Mechanisms of hyperventilation-induced lung injuries in neonatal rats
Jinjie HUANG, Benqing WU, Jinzhi SONG, Jun WU ✉
Department of Neonatal, the People’s Hospital of Shenzhen, the Second Clinical Medical College of Jinan University, Shenzhen, China
BACKGROUND: The aim of this study was to investigate the mechanism of early inflammatory injury in neonatal ventilator-induced lung injuries (VILI).
METHODS: Newborn rats were randomly assigned to groups and administrated mechanical ventilation with different tidal volumes. Morphological changes in lung tissues were observed, and the levels of interleukin-6 (IL-6), cysteinyl leukotriene mRNA (CysLT1 mRNA), and nuclear factor-κB mRNA (NF-κBp65 mRNA) in lung tissues were analyzed.
RESULTS: The ventilation groups exhibited different degrees of inflammatory cell infiltration, which was aggravated as the tidal volume and ventilation time increased. The IL-6 levels of the hyperventilation 5H, conventional ventilation 5H, hyperventilation 3H, control, and normal lung-tissue group were 785.33 ± 39.06, 701.6 ± 33.65, 686.65 ± 46.85, 637.63 ± 40.55, and 635.02 ± 65.78 pg/g, respectively. Hyperventilation increased the levels of IL-6 and NF-κBp65 mRNAas the ventilation time increased, and IL-6 was positively correlated with NF-κBp65 mRNA levels (r = 0.72, P < 0.01). Longer hyperventilation periods upregulate the level of CysLT1 mRNA. CysLT1 mRNA/GAPDH of the hyperventilation 5H group was 2.14 ± 1.45 (P < 0.01).
CONCLUSIONS: Mechanical ventilation with a large tidal volume can cause VILI, characterized at an early stage by inflammatory responses and particularly by the increased secretion and invasion of inflammatory cytokines and inflammatory cells. The activation of the NF-κB-IL-6 signaling pathway was an important mechanism for the initiation of VILI. Additionally, CysLT1 was involved in the inflammatory VILI damage, and its upregulation occurred later than that of IL-6.