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MINERVA ORTOPEDICA E TRAUMATOLOGICA
A Journal on Orthopedics and Traumatology
Minerva Ortopedica e Traumatologica 2011 April;62(2):75-81
Tendon healing in vivo: effects of antisense TGF-b1 recombinant plasmid on adhesion formation of flexor tendon
Xia C., Cao W., Wang Y., Han Y., Sun K., Tian S.
Department of Orthopedics, Hospital of Medical College, Qingdao University, Qingdao, China
Aim. Adhesion formation between the flexor tendon and its surrounding fibro-osseous sheath results in a decreased postoperative range of motion in the hand. Transforming growth factor-beta (TGF-β) is a key cytokine in the pathogenesis of tissue fibrosis. The aim of this study was to investigate the preventive effect of antisense TGF-β1 recombinant plasmid on flexor tendon adhesion formation. Thirty-six adult New Zealand White rabbits were divided into two groups randomly after anastomonsis operation of flexor tendons, as normal saline group and antisense TGF-β1 recombinant plasmid group. Tendons were harvested at 4 and 8 weeks and biomechanics testing were conducted. Forty-eight adult New Zealand White rabbit were divided into two groups randomly after anastomosis operation of flexor tendons, as normal saline group, antisense TGF-β1 recombinant plasmid group. Tendons were harvested at increasing time intervals (7, 14, 28, and 56 day) and analyzed by in situ hybridization to determine the mRNA expression of TGF-β1 and Collagen II.
Results. The results of biomechanics testing indicated that antisense TGF-β1 recombinant plasmid can prevent adhesion formation of flexor tendon effectively after anastomonsis operation. The in situ hybridization examination revealed that TGF-β1 and Collagen I mRNA expression in antisense TGF-β1 recombinant plasmid was lower than that in normal saline group at each time.
Conclusion. Antisense TGF-β1 recombinant plasmid can inhibit the function of the TGF-β1 effectively after the flexor tendon injured and repaired and prevent adhesion formatin.