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MINERVA ORTOPEDICA E TRAUMATOLOGICA
A Journal on Orthopedics and Traumatology
Minerva Ortopedica e Traumatologica 2008 April;59(2):99-107
Monolayer culture’s behaviour of articular chondrocytes and sinoviocytes to the gadolinium exposure
M. Cravino, A. Marmotti, F. Castoldi, R. Rossi, F. Giacalone
Department of Orthopaedics and Traumatology Umberto I Hospital University of Turin, Turin, Italy
The aim of this study was to find out the damaging effects on cellular
lines that are in contact with gadolinium.
Methods. This study was carried out with human chondrocytes, sinovial
cells, Hoffa’s body cells and mesenchimal cells of bone. These cells
were cultivated in monolayer for 7-14 days, exposed to gadolinium for
16 hours (1.88 mg/mL and 18.8 mg/mL), than the estimation of cellular
vitality with Trypan Blue was made, to assess cells in necrosis, and
with antibody anti-ki 67 antigen, to assess cell proliferation.
Labelled cells were counted with optical microscope in order to
estimate the mean ratio between vital cells and necrotic (blue), and
between cell in replication (positive to antibody anti-ki 67 antigen)
and cell not in replication.
Results. Present results express the inhibition of proliferation and
the induction to necrosis of Magnevist IV (Gadopentetic acid
dimeglumine salt) on cells tested. The means obtained, expressed as
index of gadolinium influence, represent the inhibition of this
substance on the singular valuated cellular line. A little induction to
necrosis and a decrease of cellular proliferation for dosing like that
used during artro-nuclear magnetic resonance was noticed. The
concentration 10 times higher, that shown great inhibition of
proliferation and induction of necrosis, allowed to corroborate the
dose-dependence and to make an ideal positive curve of exposure test.
Conclusion. Necrosis and cell proliferation suffer the consequence of