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MINERVA ORTOPEDICA E TRAUMATOLOGICA

A Journal on Orthopedics and Traumatology


Official Journal of the Piedmontese-Ligurian-Lombard Society of Orthopedics and Traumatology
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BIOMATERIALS IN ORTOPEDICS: EXPECTATION AND CLINICAL PRACTICE  96° CONGRESS OF THE PIEDMONTESE-LIGURIAN-LOMBARD SOCIETY OF ORTHOPEDICS AND TRAUMATOLOGY (SPLLOT) (Brescia, September 24-25, 1999)


Minerva Ortopedica e Traumatologica 2000 June;51(3):79-88

language: Italian

In vitro effects of biocompatible metals on the morphology, proliferation and apoptosis in cultured osteoblast lines

Massari L., Manfredini M., Casto R., Brandolini F., Colamussi M. L., Borgatti P., Neri L. M.


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Background. The success of a non-cemented prosthesis depends on its integration with the surrounding bone tissue, a phenomenon that is closely correlated to the biocompatibility of the materials used. Little is known of the effects that these biocompatible materials may have on osteoblasts, the cells that play a key function in the osteointegrating process. In particular, it is not clear what effects fragments of materials generated by wear may have on osteoblasts. These fragments are thought to play an important role in the aseptic mobilisation of prostheses. For this reason, the authors have carried out an in vitro evaluation of the interaction between commonly used biocompatible materials and cultured osteoblasts.
Methods. Flow cytofluorimetry, optic microscopy and scanning electron microscopy were used to examine the behaviour of different cell lines of human and murine osteoblasts (MG-63, SaoS-2, MC3T3) cultured in the presence of Ti-6Ai-4Va or Cr-Co-Mo alloys, corresponding to the metals that are most widely used in prostheses today. The cultures were treated for 72 h with fragments of Ti-6Ai-4Va or Cr-Co-Mo whose dimensions were divided into 2 different groups (<5 µm and 5-10 µm). The cultures were examined to evaluate morphology, adhesion, proliferation, cell cycle and apoptosis. Further investigations using an electron microscope were carried out on osteoblast cultures seeded on disks of Ti-6Ai-4Va or Cr-Co-Mo.
Results. The results obtained show that the various materials have different effects on cells, in particular Cr-Co-Mo fragments were found to be more cytotoxic than Ti-6Ai-4Va. In addition, cytotoxicity increased in relation to the size of the metal fragments.
Conclusions. Osteoblast growth was stimulated by the surface of Ti disks to a much greater extent than Cr-Co-Mo disks.

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