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MINERVA ORTOPEDICA E TRAUMATOLOGICA
A Journal on Orthopedics and Traumatology
Minerva Ortopedica e Traumatologica 1999 June;50(3):85-92
Chondrocytes growth on a biological transplantable substratum: hyaluronic acid ester. A preliminary study
Albisetti W., Sala C., Corbella M., Banfi A., La Bionda F.
Background. The authors evaluate the possibility of growth of chondrocytes on a membrane consisting completely of a hyaluronic acid ester, which is a natural polymer of the glycosamminoglycanic family, in order to identify a biological transplantable substratum which is quickly degradable and on which it is possible to cultivate a chondrocyte cellular suspension, without modifying its proliferation, and allowing a new approach to the surgical therapy of chondral defects.
Methods. 192 C57B strain rats, sacrificed at 4 days of life, were used in this experimental study. The authors, using the distal chondroepiphysis of the femur and the proximal chondroepiphysis of the tibia of the rats, made 32 chondroyte cultures. Each chondrocyte culture was then divided into 10 cultures; in 8 of them the authors introduced yaluronic acid ester, while the 2 residue cultures were used as controls, for a total of 256 cases and 64 controls.
Results. The results, after 7 days, showed regular chondrocyte growth without qualitative alterations in cellular form and dimensions. After 14 days of culture, the biomaterial filaments were no longer identifiable: the hyaluronic acid ester was completely reabsorbed by the catabolic cellular processes.
Conclusions. The experimental study gives inportant information from the results point of view, in fact hyalofill showed itself an excellent substratum for chondrocyte growth, and from the technical-methodological point of view. In fact the results are repeatable 100%.