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Online ISSN 1827-1677
Fabbri M., Venturi M., Vassallo T., Daniele M., Gaudio R. M., Avato F. M.
Laboratory of Immunology and Forensic Genetics, Section of Public Health, U.O.L of Legal Medicine, Department of Medical Sciences, University of Ferrara, Ferrara, Italy
AIM: The aim of this paper was to determine 13 new Y-STRs markers, known as rapidly mutating Y-STRs (RM-YSTRs) and represented by DYS576, DYF399S1, DYF387S1, DYS570, DYS526a, DYS526b, DYS626, DYS627, DYS518, DYS61, DYS449, DYS547, DYF404S1, DYF403S1a and DYF403S1b in 100 unrelated male individuals living in Ferrara.
METHODS: A total number of 100 unrelated Emilian males from Ferrara were used as a control population in the present study. Genomic DNA was isolated using QIAamp DNA Mini Kit (Qiagen, Limburg, The Netherlands). All PCR reactions were performed according to the conditions described by Ballantyne et al. PCR multiplex reactions were performed in a Verity® 96-Well Fast PCR System (Applied Biosystems, Waltham, MA, USA), using Qiagen Multiplex PCR kit to minimize the need of optimization. Samples were analyzed using GeneMapper ID-X 1.1 software (Applied Biosystems, Waltham, MA, USA). Population differentiation was performed using the ARLEQUIN software, V. 2.000.
RESULTS: Among the 100 individuals, gene diversity (GD) values for the population sample ranged from 0.6914 for DYS526a to 0.9914 for DYF399S1. For the 100 individuals 2 haplotypes occurred four times and the remaining 92 haplotypes were “unique”. Haplotype diversity (HD) for the 13 analyzed loci was 99.83%, discrimination capacity (DC) was 96.00% and haplotype match probability was 0.16%. The most informative locus were DYF399S1 and DYF403S1a; GD for these loci analyzed was, respectively, 99.14% and 98.78%. On the contrary, the less informative markers were DYS526a and DYS576 that showed respectively a value of GD of 69.14% and 73.80%.
CONCLUSION: This study assessed the frequency of 13 Rapidly Mutating Y-STRs (RM-YSTRs) in a sample of Italian population, represented by 100 individuals living in Ferrara. These data are useful to be applied to forensic case works and for databasing purpose.