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Online ISSN 1827-1677
Fabbri M., Venturi M., Gaudio R. M., Pasetti A., Marinelli L., Avato F. M.
Laboratory of Forensic Genetics, Section of Legal Medicine, Department of Biomedical Sciences and Advanced Therapies, University of Ferrara, Ferrara, Italy
Aim. The aim of this study was to report on the short tandem repeat analysis (STR), using PowerPlex ESX 16 Systems (Promega), of the supposed specimen belonged to M.B., who died in 1980.
Methods. DNA was extracted from three different specimen dating back to the 1950s and represented by two blood stains found on two different fragments of fabric and a blood stain found on a cotton glove. As reference sample, a bone collected from the body was used. Before DNA extraction, in order to get rid of modern DNA contaminations, the bone was treated with diluted bleach, water and UV light.
Results. The different STR profiles were confirmed using criteria currently available for validation of degraded DNA, including independent PCR replication.
Conclusion. The results obtained after the genetic analysis confirmed that the specimen analyzed, represented by two bloodstains found on two different fragments of fabric and a bloodstain found on a cotton glove, were directly referable to M.B.