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Online ISSN 1827-1677
Fabbri M., Venturi M., Ferronato C., Daniele M., Gaudio R. M., Avato F. M.
Unit of Legal Medicine, Department of Biomedics and Advanced Therapies, University of Ferrara, Ferrara, Italy
Aim. The aim of this study was the analytical and statistical evaluation of miniSTRs markers (D10S1248, D14S1434 e D22S1045) in a population sample living in Ferrara (110 unrelated individuals).
Methods. Genomic DNA was isolated using QIAamp DNA Mini Kit (Qiagen). PCR amplification was performed in a GeneAmp PCR System 2400 (Applied Biosystems), using primer sequences, reaction volumes and amplification as reported in literature. The amplified fragments were separated using a DNA sequencer ABI PRISM 377 Genetic Analyzer (Applied Biosystems). Typing of amplified alleles was carried out using the allelic ladder and the proper positive controls as internal reference according to the recommendations of the International Society of Forensic. Alleles nomenclature was updated according to the changes reported by the National Institute of Standardization and Technology (NIST). Forensic parameters (HEZ, PD, PIC, PE, TPI) were calculated using PowerStatsV1.2 software (Promega).
Results. The genotype frequencies distributions, evaluated with the Chi-square test, showed no significant deviations from the Hardy-Weinberg. The PD ranging from 0,854 (D14S1434) to 0,907 (D10S1248) while the PIC ranging from 0,650 (D14S1434) to 0,720 (D10S1248), respectively. Analysis of these parameters revealed that the most informative marker was D10S1248 and, at the contrary, the less informative was D14S1434 although showed an observed HEZ of 70%.
Conclusion. The statistical analysis revealed the efficiency of these miniSTR markers, especially for D10S1248 and D22S1045, as underlined by PIC values. MiniSTRs loci may also provide an alternative set for human identification; especially, when degradation of DNA occur these markers offer an alternative to less informative and time-consuming methods (SNPs - mitochondrial DNA).