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Minerva Medicolegale 2000 June;120(2): 65-70

language: Italian

The preparation of forensic samples for DNA analysis by PCR

Ricci U., Guarducci S., Sani I., Biondi C., Giovannucci Uzielli M. L.


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The authors propose a simple, reproducible and non-expensive protocol for the extraction and purification of biological samples found on crime scenes, which should be classified with the Polymerase Chain Reaction technique. The method allows the qualitative and semi-quantitative study of the extract. The authors report the result of the extraction, with conventional methods and with the new protocol, of some biological traces obtained from donors whose genetic profile was known, mixed with polluted agents, like plaster, asphalt and paint. With the described protocol, 22 forensic samples were analysed afterwards (blood, saliva on stamps, cigarette butts). For the validity of the method, a continuative use of the extraction method for polluted and/or degraded samples is suggested.

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