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A Journal on Internal Medicine
Indexed/Abstracted in: Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,236
Minerva Medica 2008 February;99(1):1-5
Plasma ICAM-1 and VCAM-1 levels in type 2 diabetic patients with and without microalbuminuria
Bruno C. M., Valenti M., Bertino G., Ardiri A., Bruno F., Cunsolo M., Pulvirenti D., Neri S.
Department of Internal Medicine and Systemic Diseases University of Catania, Catania, Italy
Aim. Aim of the study was to investigate plasma levels of intercellular adhesion molecule-1 (s-ICAM-1) and vascular cellular adhesion molecule-1 (s-VCAM-1) in a cohort of type 2 diabetic patients, compared to healthy control subjects, to verify whether there are differences between these two molecules and to evaluate a possible correlation with the presence of microangiopathy.
Methods. Plasma ICAM-1 and VCAM-1 levels in 49 type 2 diabetic patients, 28 microalbuminuria patients and 21 normoalbuminuria patients were investigated and compared to same levels of 15 healthy control subjects. ICAM-1 and VCAM-1 were assayed by EIA commercial kit (R&D System Co, Abington, UK), according to procedures described by the manufacturer and concentrations expressed as ng/mL. Mean ± standard deviation (SD) values for each group were compared by t test for unpaired data and Kruskall-Wallis test. Statistical significance was set at P<0.05.
Results. Mean ± SD plasma ICAM-1 was 12.96± 1.08 ng/mL in controls, 18.56±2.3 ng/mL in normoalbuminuria patients and 26.25±4.1 ng/mL in microalbuminuria patients, respectively. Mean ± SD plasma VCAM-1 was 15.96± 4.02 ng/mL in controls, 17.13±7.5 ng/mL in normoalbuminuria patients and 26.84±5.99 ng/mL in microalbuminuria patients, respectively. Statistical analysis showed a significant difference in ICAM-1 levels between controls and normoalbuminuria patients (P<0.05) and between these and microalbuminuria patients (P<0.05). VCAM-1 levels were significantly higher in microalbuminuria than in normoalbuminuria patients (P<0.05), but no significant difference was found between normoalbuminuria patients and control subjects (P>0.05).
Conclusion. Reported results show that circulating ICAM-1 is higher in microalbuminuria than in normoalbuminuria patients and also in normoalbuminuria patients than in control subjects. Circulating VCAM-1 has increased only in microalbuminuria patients. Therefore, these two molecules have different ability to assess temporal relationship between inflammatory activity and microvascular complications.