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Minerva Medica 2001 October;92(5):393-402

language: Italian

Instrument applications of three new bio-rad kits for HIV, HCV, HBSAG screening: «Combo» Genscreen Plus HIV 1/2 Ag-Ab, Monolisa anti HCV Plus V2, monolisa HBSAG Plus, with the fully authomatic system Plato 3300

Manetta T., Gay V., Spreafico G., Massaro A., Piantino P.


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One of the most common technical problems of the virological laboratories is the tests applications on the open microplates systems. Immunoenzymatic methods can present difficulties in terms of results when are apply on several instruments. We have evaluated three different new virological kits from Bio-Rad: Genscreen Plus HIV 1/2 AG-AB, Monolisa anti HCV Plus V.2 and Monolisa AgHBs Plus on the automatic system Plato 3300. The purpose of this article was to present and summarize some data on these new Bio-Rad kits designed for the screening in the Blood Banks and Virological laboratories. We followed a protocol studied for working on automatic machine with different steps using negative fresh donors sera for:
‹ study of ''edge effect'';
‹ study of specificity
and using also positive high value sera and seroconversion Bio-Rad panels for:
‹ study of contamination,
‹ study of sensitivity.
The studies showed that the Bio-Rad kits and instrument were releable, flexible, easy to use and ready for each kind of routine in Blood Banks and Virological-Microbiological centres.

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