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Indexed/Abstracted in: EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,118
Online ISSN 1827-1634
Grabmaier U. 1, 2, Rottstegge I. 1, Offers M. 1, Ziegler T. 2, 3, Brenner C. 2, Huber B. C. 2, Seissler J. 1
1 Medical Department IV, Ludwig-Maximilians-University Munich, Campus Innenstadt, Munich, Germany;
2 Medical Department I, Ludwig-Maximilians- University Munich, Campus Großhadern, Munich, Germany;
3 Department of Molecular Medicine, Max-Planck-Institute of Biochemistry, Munich, Germany
AIMS: Recent studies suggest that stem cells may represent a putative source for the generation of beta cells. However, the identity and characteristics of stem cells from adult pancreas and conditions for their large scale expansion are still poorly defined.
MATERIALS AND METHODS: DPC were isolated from adult pancreatic ducts of C57Bl/6 mice. Expression profile was investigated by PCR, FACS and immunohistochemistry.
RESULTS: DPC express a panel of stem cell associated markers such as Pdx-1, cytokeratin-19 (CK19), Nestin, Sox9 together with the transcription factor MafA and hepatic nuclear factors HNF1β, HNF3β, HNF4α und HNF6. This gene expression profile is suggesting that DPC might be a promising tool for endocrine differentiation. After stimulation with picolinic acid and hypoxia, DPC expressed the endocrine differentiation marker Ngn3. Nevertheless, insulin production was not observed.
CONCLUSION: We here describe a protocol for the isolation end expansion of murine pancreatic ductal progenitor cells (DPC) displaying high self-renewal, spheroid- and colony-forming capacity. Further studies are required to elucidate the conditions for differentiation into mature pancreatic endocrine cell lineages.