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Home > Journals > Minerva Endocrinologica > Past Issues > Minerva Endocrinologica 2001 December;26(4) > Minerva Endocrinologica 2001 December;26(4):277-84

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CURRENT ISSUEMINERVA ENDOCRINOLOGICA

A Journal on Endocrine System Diseases

Indexed/Abstracted in: EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,118

Frequency: Quarterly

ISSN 0391-1977

Online ISSN 1827-1634

 

Minerva Endocrinologica 2001 December;26(4):277-84

NEW DIAGNOSTIC AND THERAPEUTIC STRATEGIES: SOMATOSTATIN ANALOGS. Part II 

The activation of the phoshotyrosine phosphatase h is responsible for the somatostatin inhibition of PCCL3 thyroid cell proliferation

Florio T., Arena S., Thellung S., Iuliano R., Corsaro A., Massa A., Villa V., Pattarozzi A., Diana F., Fusco A., Schettini G.

Background. This study was aimed to identify possible intracellular effectors of the somatostatin (SST) antiproliferative activity, in PCCl3 thyroid cells.
Methods. To prove the involvement of r-PTPh in SST's effect, we studied th proliferative activity of subclones of PCCl3 cells that do or do not express this PTP.
Results. SST inhibited PCCl3 TSH+insulin-dependent cell proliferation through the induction of a phosphotyrosine phosphatase (PTP) activity, detected using the synthetic substrate pNPP (+150%, p<0.01). Conversely, PCCl3 cells stably expressing the v-mos oncogene (PCmos) were completely insensitive to SST antiproliferative effects due to the incapability of SST to increase PTP activity, that correlated with the abolishment of the expression of the receptor-like PTP, r-PTPh. In the cells in which r-ptPh was transfected (PCmos/ PTPh) SST inhibited cell proliferation showing a dose-dependence similar to that observed in PCCl3 cells. Conversely, the transfection of a catalytically inactive mutant of r-PTPh did not restore the responsivity to SST. Also in PCmos/PTPh cells SST, treatment increased membrane PTP activity.
Conclusions. SST inhibition of PCC13 cell proliferation requires the activation of r-PTPh

language: Italian


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