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A Journal on Endocrine System Diseases
Indexed/Abstracted in: EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
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Minerva Endocrinologica 2000 June;25(2):33-8
Nitrite, endothelin and E-selectin plasma levels after arginine infusion in normal, obese and type 2 diabetic subjects
Ferlito S., Gallina M.
Background. The authors studied the behaviour of nitrites (specific end-products of nitric oxide), endothelin and E-selectin after arginine infusion (biosynthetic precursor of nitric oxide).
Methods. The series consisted of 28 female subjects (mean age 59±8) of whom: 12 normal controls; 12 suffering from essential obesity (BMI 31.4±0.7); 14 suffering from uncomplicated type 2 diabetes mellitus.
Each subject received intravenous arginine (20 g in two hours); before the infusion and after 1 hour and 2 hours venous blood withdrawal was performed. For each plasma sample the levels of nitrites (colorimetric reaction), endothelin (ELISA method) and E-selectin (ELISA method) were determined.
Results. In the controls plasma nitrites decreased significantly (p<0.05), more evidently at the second hour. In the obese subjects a sharp significant (p<0.05) fall of nitrites was observed, more evidently at the second hour. In the diabetics nitrite values were reduced (p<0.05) compared to the baseline.
Endothelin levels after arginine were almost unchanged in normal and obese subjects, while in diabetics a significant (p<0.05) reduction was observed. In the three groups of subjects E-selectin values were not modified by arginine.
Conclusions. This study demonstrates that arginine infusion increase NO biosynthesis in normal, obese and diabetic subjects, inducing a significant reduction of nitrites for an early process of organic reconversion; endothelin showed a reductive trend, mainly in diabetics, probably due to an antagonistic balanced response towards an overproduction of nitric oxide. E-selectin did not change, without correlation with the nitrite and endothelin values, because of an independence from the production of the two ''hemodynamic markers''.