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A Journal on Biotechnology and Molecular Biology

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Minerva Biotecnologica 2017 March;29(1):30-2

language: English

Biological synthesis of Au-Ag alloy nanoparticles using Escherichia coli


Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran


BACKGROUND: We reported an environment friendly method for biosynthesizing nanoscale Au-Ag alloy using the culture supernatant of Escherichia coli.
METHODS: Aqueous solutions of chloroaurate and AG+ ions were treated with the culture supernatant of E. coli for the formation of AU-AG alloy nanoparticles. The reaction between this supernatant and AG+: AU3+ mixtures ions were carried out for 20 minutes at room temperature.
RESULTS: In order to identify and analyze nanoparticles, UV-VIS spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS) were used. In this research, it was found that alloy AU-AG nanoparticles are synthesized in a size range between 6-12 NM.
CONCLUSIONS: We described an economical method for reducing AG+: AU3+ mixtures ions to produce nanoparticles. AU-AG bimetallic nanoparticles in the range of 6-12 NM are synthesized by the supernatant of E. coli. The process of reduction is extracellular, which makes it an easier method for the synthesis of AU-AG alloy nanoparticles.

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