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Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Online ISSN 1827-160X
Ahmad GHOLAMI 1, 2, Sara RASOUL-AMINI 3, Alireza EBRAHIMINEZHAD 2, 4, Narjeskhatoon ABOOTALEBI 2, Uranous NIROUMAND 3, Narges EBRAHIMI 1, 2, Younes GHASEMI 1, 2
1 Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; 2 Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; 3 Department of Medicinal Chemistry, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; 4 Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
BACKGROUND: Recently, magnetic nanoparticles have been a great focus of research due to their many applications from medicine to industry. For biomedical purposes, it is desirable to modify the surface of magnetic nanoparticles by biocompatible molecules. Although there are numerous studies about antibacterial activity of metal nanoparticles, only limited studies have been published about the effect of iron-oxide nanoparticles (IONs) on the growth of microorganisms.
METHODS: In this study, naked, glycine and two different lipoamino-acid-coated iron oxide nanoparticles have been synthesized and characterized by X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometer and differential scanning calorimeter techniques. subsequently, in order to evaluate antibacterial effects of these particles, impacts of them was studied on growth of six Gram-positive and Gram-negative microbial strains including Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, Shigella flexneri, Escherichia coli, Pseudomonas aeruginosa by the microdilution method.
RESULTS: The results revealed that all nanoparticles were dose-dependently affecting microbial growth. At high concentrations (200 and 100 µg/mL), there was antimicrobial activity. Reversely, at low concentration (10 and 1 µg/mL), a growth promotion effect was observed. Among our nanoparticles, 2-amino-octadecanoic acid coated iron-oxide nanoparticles (LION16) presented greatest antibacterial effect against both Gram-positive and Gram-negative bacteria used in this study.
CONCLUSIONS: The obtained results suggested that the antimicrobial activity of surface modified IONs is dependent on the size of nanoparticles, type of coating, and their concentrations. It was found that LION16 have remarkable bactericidal potential, and on the other hand, 2-amino-octadecanoic acid coated iron-oxide nanoparticles showed the best performance on induction of bacterial growth.