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A Journal on Biotechnology and Molecular Biology
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Minerva Biotecnologica 2016 March;28(1):29-32
Microbial synthesis of gold nanoparticles with Salmonella typhirium
Hamid R. GHORBANI
Department of Chemical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran
BACKGROUND: Green synthesis of gold nanoparticles is important because of their biocompatibility, low toxicity, and eco-friendly nature. In the present study, we describe a cost effective and eco-friendly approach for green synthesis of gold nanoparticles from 1 mm haucl4 solution using the bacterium Salmonella typhirium.
METHODS: In this work, Au nanoparticles are synthesized by culture supernatants of Salmonella typhirium. The Trypti casein soy broth (TSB) medium was prepared, sterilized and inoculated with a fresh growth of Salmonella typhirium. After incubation the supernatant was used for subsequent experiments.
RESULTS: In order to identify and analyze nanoparticles, UV-vis spectroscopy, transmission electron microscopy (Tem), and dynamic light scattering (DLS) were used. The synthetic process was fast, and gold nanoparticles were formed within six min of gold ion coming in contact with the cell filtrate. The absorption spectra of colloidal gold nanoparticles showed characteristic surface plasmon resonance (SPR) peak centered at a wavelength of 550 nm. The results showed that mean diameter and standard deviation for the formation of gold nanoparticles were 45±10 nm.
CONCLUSION: In this work the nanoparticles in the range of ~45 nm are synthesized by the supernatant of Salmonella typhirium when it is added to haucl4. It seems this method is the fastest biological method to form Au nanoparticles.