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Minerva Biotecnologica 2015 December;27(4):201-9

language: English

Molecular evaluation of wheat genotypes for vernalization response based on the Intron 1 deletion in VRN gene

Ullah M. W., Hussain J., Ahmad R., Hassan A., Shah M. M.

Biotechnology Program, Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan


AIM: The current study was aimed to evaluate the winter wheat lines for its potential of vernalization in semitemperate conditions. Besides, allelic variation of vernalization (VRN-1) gene at promoter region was also analyzed.
METHODS: Analysis of variance and Pearson correlation were applied to investigate the relationship between grain yield and agronomic traits. Genotypes were analyzed with PCR-based VRN-1 gene and allele-specific markers to investigate the likely presence or absence and allelic variation of VRN-1 gene.
RESULTS: It was found that tillers (TLL) and plant height (PH) were positively correlated with yield, while flag leaf sheath (FLS), awn length (AL), flag leaf area (FLA) and peduncle length (PL) were less positively correlated with yield. Similarly, stem diameter (SD), dry biomass (DB), lodging (LOD) and grain weight (GW) were negatively correlated with yield. Intron-1 deletion associated with spring growth habit was observed at the VRN-B1 loci of VRN-1 gene in four-wheat genotypes (WWline16, WWline42, WWline56, and WWline107). No intronic deletions were observed at the VRN-1 loci of either genome in three wheat genotypes (WWline46, WWline75 and WWline109) and thus followed winter growth habit.
CONCLUSION: Correlation analysis of traits and molecular evaluation of lines will help architecting wheat genotypes suitable for the temperate and semi temperate conditions.

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