Advanced Search

Home > Journals > Minerva Biotecnologica > Past Issues > Minerva Biotecnologica 2015 June;27(2) > Minerva Biotecnologica 2015 June;27(2):93-8

ISSUES AND ARTICLES   MOST READ   eTOC

CURRENT ISSUEMINERVA BIOTECNOLOGICA

A Journal on Biotechnology and Molecular Biology

Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246

Frequency: Quarterly

ISSN 1120-4826

Online ISSN 1827-160X

 

Minerva Biotecnologica 2015 June;27(2):93-8

    ORIGINAL ARTICLES

Optimization of medium for biological potency of fermentation supernatant of Paenibacillus brasilensis YS-1

Tu Q. 1, Zhao H. 2, Chen J. 3

1 Soil and Fertilizer Technology Extension Station of Jiangxi Province, Nanchang, China;
2 College of Economics and International Trade, Zhejiang University of Finance & Economics, Hangzhou, China;
3 College of Agronomy, Jiangxi Agricultural University, Nanchang, China

AIM AND METHODS: Medium components were optimized using one-factor-at-a-time and orthogonal array method in this study. With biological potency as index, the best carbon source and nitrogen source respectively were soluble starch and yeast extracted through one-factor-at-a-time test. Subsequently, the concentration of soluble starch, yeast extract and mineral sources were optimized using the orthogonal array method.
RESULTS AND CONCLUSION: The results showed that the optimal culture medium was 7.0% soluble starch, 2.5% yeast extract, 0.6% NaCl, 0.2% MgSO4 and 0.2% K2HPO4, and the order of factor effects on biological potency of supernatant of strain YS-1 was soluble starch >MgSO4 > yeast extract >K2HPO4 >NaCl. In addition, the confirmation experiments determined the validity of the method. Biological potency of supernatant of strain YS-1 in optimized media by orthogonal array methods was about 2.53 times greater than higher than that of one-factor-at-a-time experiment, and 4.52 times greater than with the basal medium.

language: English


FULL TEXT  REPRINTS

top of page