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Minerva Biotecnologica 2015 June;27(2):93-8

language: English

Optimization of medium for biological potency of fermentation supernatant of Paenibacillus brasilensis YS-1

Tu Q. 1, Zhao H. 2, Chen J. 3

1 Soil and Fertilizer Technology Extension Station of Jiangxi Province, Nanchang, China;
2 College of Economics and International Trade, Zhejiang University of Finance & Economics, Hangzhou, China;
3 College of Agronomy, Jiangxi Agricultural University, Nanchang, China


AIM AND METHODS: Medium components were optimized using one-factor-at-a-time and orthogonal array method in this study. With biological potency as index, the best carbon source and nitrogen source respectively were soluble starch and yeast extracted through one-factor-at-a-time test. Subsequently, the concentration of soluble starch, yeast extract and mineral sources were optimized using the orthogonal array method.
RESULTS AND CONCLUSION: The results showed that the optimal culture medium was 7.0% soluble starch, 2.5% yeast extract, 0.6% NaCl, 0.2% MgSO4 and 0.2% K2HPO4, and the order of factor effects on biological potency of supernatant of strain YS-1 was soluble starch >MgSO4 > yeast extract >K2HPO4 >NaCl. In addition, the confirmation experiments determined the validity of the method. Biological potency of supernatant of strain YS-1 in optimized media by orthogonal array methods was about 2.53 times greater than higher than that of one-factor-at-a-time experiment, and 4.52 times greater than with the basal medium.

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