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Minerva Biotecnologica 2015 June;27(2):77-83

language: English

Comprehensive quality evaluation of Chi Shao by HPLC

Jian Z. 1, Wang W. 2, 3, Xu G. 1, Meng L. 1, Hou J. 3

1 Henan Institute of Science and Technology, Xinxiang, China;
2 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, China;
3 School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China


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AIM: The purpose of this paper was to comprehensively evaluate the quality of Chi Shao.
METHODS: In the experiment of this paper, the fingerprint spectrums of Chi Shao in all locations are established by RP-HPLC and the model of principle component analysis with the RP-HPLC peak areas is established.
RESULTS: The quality of Chi Shao in the northern part of China or that made of Paeonia lactiflora is better than that of these in others or that made of other species. The quality of Chi Shao comes from P. veitchii is in the middle class and is better than those that comes from P. obovata, P. mairei and P. anomala. The results are consistent with traditional views of the quality of this plant. These results indicates that principal component analysis (PCA) can be used as an effective and economic method to evaluate the quality of Chi Shao, and may be extended to other Chinese medicinal plants.
CONCLUSION: Due to the complex basis of the efficacy of Traditional Chinese Medicine (TCM), the method such as PCA of several chemical components appears to be a more appropriate method for the quality evaluation of TCM in contrast to the determination of a single or few chemicals.

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