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A Journal on Biotechnology and Molecular Biology
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Minerva Biotecnologica 2015 June;27(2):71-5
Effect of boric acid and sucrose concentration on seedling plantlet characteristics of tomato (sopra) in in-vitro culture
Atashi S. 1, Mashayekhi K. 1, Daraei Garmakhany A. 2, Khodabakhshi A. H. 3
1 Department of Horticultural Science, University of Agricultural Science and Natural Resources, Gorgan, Iran;
2 Department of Food Science and Technology, Toyserkan Faculty of Industrial Engineering, Bu-Ali Sina University, Hamadan, Iran;
3 Department of Agronomy Science, University of Agricultural Science and Natural Resources, Gorgan, Iran
AIM: In order to control of environmental condition, in-vitro culture is a suitable system to biological investigation in plants. There are few reports in mutual effect of borate and sucrose in plant physiology. this information is limited to how carbohydrates are transported in combination with borate. Based on the incidental observation during our previous research about embryogenesis in carrot the hypothesis that borate element is a sucrose conveyer not only in plant tissue but also has this ability out of plants tissue was proposed. Lycopersicom esculentum has an important role in supplying vitamins and nutrition in human body. In this study mutual effect of borate and sucrose were investigated under in-vitro laboratory culture in four replications.
METHODS: Treatments included borate and sucrose in different concentrations in B5 media.
RESULTS: Results showed that borate facilitates sucrose transportation in tissue and influences sucrose transfer inside tissue. Simultaneous application of borate and sucrose significantly increased length and weight of stem and root. Between treatments the highest amount of chlorophyll (2.412 mg/fresh weight) was observed in sucrose treatment. The highest value of total carbohydrate in stem was observed in sucrose and borate treatment with amount of 1.736 and 1.469 (g/fresh stem weight) respectively.
CONCLUSION: This study showed that simultaneous application of sucrose and borate formed a combination which could transfer carbohydrates from outside to inside parts.