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A Journal on Biotechnology and Molecular Biology
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Minerva Biotecnologica 2015 March;27(1):51-4
Prevalence of Panton-valentine gene in Staphylococcus aureus isolated from clinical samples and healthy carriers in Gorgan city, north of Iran
Kamarehei F., Rahimi-Alang S., Vaez H., Ghaemi E.
Microbiology Department and Infectious Disease Research Center, Golestan University of Medical Sciences, Golestan, Iran
AIM: Staphylococcus aureus (S. aureus) is a nosocomial and community acquired pathogen. S. aureus is a pathogen that causes several types of disease from skin infections to systemic diseases that is because of having several virulence factors such as enzymes, toxins, superantigens and Panton-Valentine leukocidin (pvl). pvl is a bi-component leukotoxin that destroy PMNs and monocytes and causes furunculosis, abscesses and necrotizing soft tissue infections in patients without any risk factors for such infections. The goal of this study was determine the prevalence of pvl gene in S. aureus isolated from patients and healthy carriers in Gorgan city, north of Iran.
METHODS: One hundred seventy isolates of S. aureus, 95 from patients and 75 healthy carriers, were collected during one year. After identification and purification, DNA extraction was done by phenol-chloroform method. Amplification of pvl gene was done by specific primer and polymerase chain reaction method.
RESULTS: Among the 170 isolates of S. aureus, 20% contained pvl gene. The frequency of isolates contained pvl gene in MRSA and MSSA isolates were 21.6%, 19.3% , which was not statistically significant. The frequency of these genes was not related to age, sex and source of isolation from patients.
CONCLUSION: The frequency of pvl gene in this region were much higher than expected.