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A Journal on Biotechnology and Molecular Biology
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Minerva Biotecnologica 2014 December;26(4):215-21
Inhibiting the growth of pathogenic microorganisms and producing flavonoids from endophytic fungi associated with Scutellaria baicalensis
Cui J. L. 1, 2, Fan L. 1, Fu S. B. 3, Wang M. L. 2, Zhang N. S. 2, Guo T. T. 2
1 College of Life Science, Capital Normal University, Beijing, China;
2 Institute of Applied Chemistry, Shanxi University, Taiyuan, China;
3 Pharmacy School of Zunyi Medical College, Zunyi, China
AIM: The aim of this paper was to investigate the flavonoid-producing endophytic fungi from 102 strains from S. baicalensis and to evaluate their antimicrobial activities.
METHODS: Isolation of endophytic fungi was adopted by the tissue method and fungal identification were based on their rDNA-ITS sequences. Abilities of fungi to synthesizing baicalin, baicalein, or wogonin was used the technologies of thin-layer chromatography and high-performance liquid chromatography and the agar well diffusion method was used to evaluate their ability to inhibit microbe growth.
RESULTS: It was found that four fungi, C.SBA7, C.SBO7, C.SBA4, and N.SBA11 belong to Fusarium sp., Phoma sp., Alternaria sp., and Fusarium sp., respectively, based on their internal transcribed spacer region r-DNA sequences, have the ability to synthesize baicalin, baicalein, or wogonin associated with S. baicalensis. All of them showed anti-microbial activities against at least one pathogenic microorganism in test.
CONCLUSION: This study has provided a strategy for producing baicalin, baicalein, or wogonin which were originally produced from S. baicalensis in a sustainable way by using the fungal fermentation, and also provided the biological data for the four fungi.