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A Journal on Biotechnology and Molecular Biology
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Minerva Biotecnologica 2014 September;26(3):183-9
Chemical composition and biological activities of Scrophularia striata extracts
Akbar Jafari A. 1, Shohrati M. 2, Mahmoudi R. 3, Haj Hoseini R. 4, Nosratpour S. 3, Pajohi-Alamoti M. 5, Mohamad Latifi A. 6
1 Department of Biology, Faculty of Science, Payam Noor University, Tehran, Iran;
2 Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;
3 Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;
4 Department of Biochemistry, Faculty of Science, Payam Noor University, Tehran, Iran;
5 Department of Food Hygiene, Faculty of Para‑Veterinary Sciences, Bu‑Ali Sina University, Hamedan, Iran;
6 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
AIM: The aim of this study was to investigate chemical composition (GC/MS analysis), antioxidant (DPPH) and antibacterial (MIC analysis) activities of three extracts obtained from Scrophularia striata.
METHODS: The extraction of Scrophularia striata plant was extracted through ethanolic, hydro alcoholic and aqueous methods. The chemical composition of the three extracts is determined by GC-mass analysis method. DPPH assay was performed for measurement of antioxidant activity. MIC and MBC were determined with micro well dilution assey.
RESULTS: GC-MS analysis revealed that ethanolic and aqueous extracts were rich in Bis(2-ethylhexyl) phthalate (25.17%, 27.855% respectively) and hydro alcoholic extract in Oleyl Alcohol (24.81%). All of the extracts had the antibacterial activity. Among all extracts, hydro alcoholic extract indicated the most activity against pathogenic bacteria, and had high antioxidant activities as measured by DPPH scavenging (0.407 mg/mL). The aqueous extract had the lowest amount of total phenolic compounds (88.42±2.55 mg).
CONCLUSION: A strong correlation was observed between the antibacterial, radical scavenging capacity and the phenolic and flavonoid compounds of the extracts. As expected, amounts of the total phenolic and flavonoid components were very high in the hydro alcoholic extract.