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A Journal on Biotechnology and Molecular Biology

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Minerva Biotecnologica 2013 December;25(4):227-34

language: English

Saccharomicies cerevisiae yeast growth conditions optimisation using RSM methodology for the production of β-glucan

Javmen A., Grigiškis S., Rudenkov M., Pelisauskaitè E.

JSC “Biocentras”, Vilnius, Lithuania


Aim: β-glucan is polymeric compound in which glucose monomers are linked by β glycosidic bonds. β-glucan is produced by many different organisms – bacteria, fungi, plants. It was noted that different β-glucans can effect mammals immunity and can be human immune system modulators. Many experiments showed that β-glucans can be possibly used in bacterial disease therapy and can increase immune system cells cytotoxicity against cancer tumors. An important source of immune active β-glucan is baker’s yeast Saccharomicies cerevisiae: one of the major and structurally important component of S. cerevisiae yeast cell wall component is β-(1-3, 1-6) glucan. For baker’s yeast cultivation, YEPD medium is often used, which consists of glucose, yeast extract and peptone. The aim of this work was to use usual pabular low-cost glucose for Saccharomicies cerevisiae fermentations and optimizate cultivation process conditions for maximal β-glucan yield extraction from yeast cells.
Methods: To reach this aim response surface methodology (RSM) was used. Alternate medium was also used, for cultivation of Saccharomicies cerevisiae, which consists of low-cost glucose and corn steep liquor; yeast cultivation process optimization for maximum yield of β-glucan was undertaken.
Results: Optimal conditions were determined and verified for both two medium types.
Conclusion: Both optimized mediums can be practical used for a β-glucan production.

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