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A Journal on Biotechnology and Molecular Biology
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
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Minerva Biotecnologica 2012 September;24(3):111-5
Enhancement of gluconic acid pathway in A. niger RP-11 by mixed chemicals method
Geetha P. 1, Raksha S. 2, Prabu R. 3
1 Department of Biochemistry, M.R Government College, Mannargudi, India;
2 Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia;
3 Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, UPM Serdang, Selangor, Malaysia
Aim. The aim of the study was amend the sodium gluconate producing strain the Aspergillus niger RP-11 by mixed chemical method.
Methods. Mixed chemical mutagens mixture (2 mg/mL) such as N-Methyl-N’ nitro-N-nitrosoguanidine (NTG), Methyl methane sulfonate (MMS), ethyl methane sulfonate (EMS) were applied for amend the bioconversion of sodium gluconate from glucose suspension. This mixed chemical mutagen with changes of glucose oxidase enzyme activity and sodium gluconate concentration were studied /before the mutation.
Results. Out of 10 overproducing mutants tested in shaken flasks, A.niger RP-MCM-51 showed the highest level of gluconate production (2.5 fold higher than the wild-type). The mutant strain A.niger RP-MCM-51 has showed the 3.8 fold higher glucose oxidase activity during the growth in fermentation on 380-420 hrs. Spores of the mutants were stable and have consistent ability in producing sodium gluconate after prolonged storage in glycerol at -20° C.
Conclusion. The mutant strain and the wide-eyed simple method are useful to development of fermentation process for industrial production of gluconic acid or its salt.