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CURRENT ISSUEMINERVA BIOTECNOLOGICA

A Journal on Biotechnology and Molecular Biology

Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246

Frequency: Quarterly

ISSN 1120-4826

Online ISSN 1827-160X

 

Minerva Biotecnologica 2008 December;20(4):165-74

New trends in chemistry and biotechnology of peptide nucleic acidS 

Surface plasmon-based optical detection of DNA by peptide nucleic acids

Zanoli L. 1, D’Agata R. 1, Spoto G. 1,2

1 Department of Chemical Sciences University of Catania, Catania, Italy
2 Institute of Biostructures and Bioimages CNR, Catania, Italy

Surface plasmon-based optical methods using peptide nucleic acids (PNAs) probes have recently emerged as powerful and highly sensitive tools for DNA and RNA detection. Surface plasmon resonance (SPR) is a powerful technique for the high sensitive and label-free detection of nucleic acids while PNAs help in targeting complementary sequences with exceptionally high selectivity and sensitivity. The present review summarizes the research efforts paid over the last years in the above mentioned field. In particular, fundamental aspects of the SPR detection of the PNA/DNA hybridization will be discussed. Then, research activities aimed at demonstrating the ultrasensitive DNA detection and the possibilities offered in single nucleotide polymorphisms detection will be presented. The contribution given by surface plasmon-allied techniques and by the combined use of the electrochemical detection will be also discussed. The SPR detection of DNA by chiral PNAs will be finally mentioned.

language: English


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