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A Journal on Biotechnology and Molecular Biology

Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246

Frequency: Quarterly

ISSN 1120-4826

Online ISSN 1827-160X


Minerva Biotecnologica 2006 March;18(1):39-49



A new technique for biotechnological products loading into microparticles

Estevan M. 1,2, García Del Barrio G. 1, Gamazo C. 2, Novo F. J. 3, Irache J. M. 1

1 Centro Galénico, Department of Pharmaceutical Technology University of Navarra, Pamplona, Spain
2 Department of Microbiology University of Navarra, Pamplona, Spain
3 Department of Genetics University of Navarra, Pamplona, Spain

Aim. Biodegradable microparticles have been extensively and effectively used as controlled release systems of bioactive materials.
Methods. One of the most widely used methods for the preparation of biodegradable polymer microparticles is the double-emulsion solvent-evaporation method. However, the emulsification techniques based on homogenization procedures can be harmful to the integrity and bioactivity of the substance. The preservation of stability is of great importance in many circumstances: i.e., in the case of a vaccinal application, the native form is required to stimulate the right cell receptors to induce the appropriate immune response; and in gene therapy, the integrity is important to induce a significant gene expression. The aim of this work was to evaluate the potential of a new method named TROMS (Total Recirculation One-Machine System) for the encapsulation of several unstable materials such as recombinant adenovirus, DNA plasmids and antigenic complexes avoiding the use of aggressive homogenization techniques.
Results. This system minimizes the possibility of manipulation mistakes and diminishes the shearing forces. Moreover, the control of the processing parameters permits to select the size of resulting microparticles, the preparation of large batches and to work in a continuous way.
Conclusion. TROMS appears to be well suited for the encapsulation of sensible biotechnological products, such as plasmid DNA, adenovirus and proteins with antigenic properties.

language: English


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