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Minerva Biotecnologica 2005 September;17(3):133-9

language: English

Factors affecting root growth and metabolite production in Salvia miltiorrhiza adventitious root cultures

Guo X. 1, Gao W. 1, Xiao P. 2

1 College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin, China;
2 Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

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Aim. Root growth and secondary metabolite formation in Salvia miltiorrhiza adventitious root cultures were affected by factors including sucrose concentration, plant growth regulators, nitrogen sources and ultrasound.
Methods. Under various sucrose concentrations, the maximum biomass and production of tanshinone IIA and protocatechuic aldehyde were obtained with 60 g·L-1, 20 g·L-1, 30 g·L-1 sucrose in culture medium, respectively. Plant growth regulator combinations of IBA and KT had different effects in this culture.
Results. The results indicated that the highest growth rate, i.e. 4.17 by dry weight, a relatively high productivity of tanshinone IIA and protocatechuic aldehyde, being 0.19 mg·g-1 and 0.32 mg·g-1, respectively, were reached with combinations of the growth regulators of IBA 2-KT 0, IBA 2-KT 1 and IBA 0-KT 0.5, respectively. When adding various ratios of NH4+/NO3– to the medium and keeping the total initial nitrogen source to 60 mmol·L-1, a relatively high biomass and maximum secondary metabolite production were achieved with nitrate as the sole nitrogen source.
Conclusion. As for ultrasound-induction, higher power level, i.e. 300 W and 500 W for long exposed time (3-4 min) inhibited root growth.

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