Total amount: € 0,00
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Online ISSN 1827-160X
Gardenghi S. 1, Finotti A. 1,2, Gambari R. 1, 2, Feriotto G. 1
1 Biotechnology Center, University of Ferrara, Ferrara, Italy;
2 Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy
The analysis of polymerase-chain reaction (PCR) products by surface plasmon resonance (SPR) -based biospecific interaction analysis (BIA) using biosensors could be an excellent tool for specific and sensitive detection of genetically modified organisms (GMO). While this technology has several important advantages, a major drawback is that, in some instances, secondary structures of the PCR products to be analysed lead to low efficiency in binding to the specific probes. Cross-hybridization between PCR products and probes could also occur, especially when standard BIA conditions are employed. Here we demonstrate that biospecific interaction analysis, employing surface plasmon resonance and biosensor technologies, is suitable to detect Bt-176 “maximizer” sequences in maize. Not all the probes and PCR products were found to be suitable for SPR-based BIA. However, we designed and characterized probes able to selectively identify and discriminate Bt-176 and zein gene sequences exhibiting high hybridisation efficiency and no cross-hybridization. The results obtained suggest that SPR-based BIA is a flexible, easy, speedy and automatable approach to detect genetically modified Bt-176 maize in foods.