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Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
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TRENDS IN MOLECULAR DIAGNOSIS AND THERAPY OF β-THALASSEMIA AND SICKLE CELL ANEMIA
Breveglieri G. 1,2, Gardenghi S. 2, Gambari R. 1, 2, Feriotto G. 1
1 Biotechnology Center, University of Ferrara, Ferrara, Italy;
2 Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy
In the present review we describe a new methodology employing surface plasmon resonance (SPR) and biosensor technology to detect point mutations, including those causing a severe form of β°IVSI-1 thalassemia. The data presented allow to conclude that biospecific interaction analysis (BIA) is a fast and automatable approach for detecting mutations of the β-globin gene by the real-time monitoring of hybridization between oligonucleotide (ODN) probes and target biotinylated polymerase-chain reaction (PCR) products generated from genomic DNA of normal, heterozygous subjects and homozygous β°-thalassemia patients and immobilized on streptavidin coated sensor chips. During the association phase, discrimination between mismatched and full matched ODN/PCR product hybrids is readily and reproducibly observed.