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  MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002
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Minerva Biotecnologica 2002 December;14(3-4):265-7

Copyright © 2003 EDIZIONI MINERVA MEDICA

language: English

Ligase detection reaction and universal array as a tool to detect grapevine infecting phytoplasmas

Frosini A. 1, Casati P. 4, Bianco P. A. 4, Bordoni R. 2, Consolandi C. 1, Castiglioni B. 3, Mezzelani A. 2, Rizzi E. 2, Battaglia C. 1, Belli G. 4, Rossi Bernardi L. 1, De Bellis G. 2

1 Department of Biomedical Technologies and Sciences, University of Milan, LITA, Segrate (Milano), Italy; 2 Institute of Biomedical Technologies, National Council for Scientific Research, Milan, Italy; 3 Institute of Agricultural Biotechnology and Biology, National Council for Scientific Research, Milan, Italy; 4 Institute of Plant Pathology, University of Milan, Milan, Italy


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Phy­to­plas­mas are ­unculturable wall ­less bac­te­ria ­involved in ­many ­plant dis­eas­es; in par­tic­u­lar Fla­ves­cence ­dorée rep­re­sents a ­severe ­threat to vit­i­cul­ture, ­because of its epidemic behavior in field. ­Here we ­present the appli­ca­tion of the LDR tech­nique, asso­ciat­ed ­with hybrid­isa­tion on uni­ver­sal ­arrays, as a ­quick and reli­able ­tool for ­plant qua­ran­tine pro­ce­dures and man­da­to­ry meas­ures, in ­order to dis­tin­guish ­between dif­fer­ent ­phytoplasmas’ ­induced infec­tions, com­pared to clas­si­cal pro­to­cols in use, par­tic­u­lar­ly PCR - ­RFLP ­assays.

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