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  MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002


Minerva Biotecnologica 2002 December;14(3-4):265-7

language: English

Ligase detec­tion reac­tion and uni­ver­sal ­array as a ­tool to ­detect grape­vine infect­ing phy­to­plas­mas

Frosini A. 1, Casati P. 4, Bianco P. A. 4, Bordoni R. 2, Consolandi C. 1, Castiglioni B. 3, Mezzelani A. 2, Rizzi E. 2, Battaglia C. 1, Belli G. 4, Rossi Bernardi L. 1, De Bellis G. 2

1 Depart­ment of Bio­med­i­cal Tech­nol­o­gies and Sci­enc­es, Uni­ver­sity of ­Milan, ­LITA, Segrate (Mila­no), Ita­ly;
2 Insti­tute of Bio­med­i­cal Tech­nol­o­gies, Nation­al Coun­cil for Sci­en­tif­ic ­Research, ­Milan, Ita­ly;
3 Insti­tute of Agri­cul­tu­ral Bio­tech­nol­o­gy and Biol­o­gy, Nation­al Coun­cil for Sci­en­tif­ic ­Research, ­Milan, Ita­ly;
4 Institute of ­Plant Pathol­o­gy, Uni­ver­sity of ­Milan, ­Milan, Ita­ly


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Phy­to­plas­mas are ­unculturable wall ­less bac­te­ria ­involved in ­many ­plant dis­eas­es; in par­tic­u­lar Fla­ves­cence ­dorée rep­re­sents a ­severe ­threat to vit­i­cul­ture, ­because of its epidemic behavior in field. ­Here we ­present the appli­ca­tion of the LDR tech­nique, asso­ciat­ed ­with hybrid­isa­tion on uni­ver­sal ­arrays, as a ­quick and reli­able ­tool for ­plant qua­ran­tine pro­ce­dures and man­da­to­ry meas­ures, in ­order to dis­tin­guish ­between dif­fer­ent ­phytoplasmas’ ­induced infec­tions, com­pared to clas­si­cal pro­to­cols in use, par­tic­u­lar­ly PCR - ­RFLP ­assays.

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