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A Journal on Biotechnology and Molecular Biology


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Minerva Biotecnologica 2001 December;13(4):287-93

DNA MICROARRAY TECHNOLOGIES MEETING 

Antigen micro­ar­rays for the serod­i­ag­no­sis of infec­tious dis­eas­es

Marcolini S.

SEAC/Radim SpA, Pomezia, Roma

Microscopic ­arrays (micro­ar­rays) of immob­il­ised ­reagents on sol­id sur­fac­es in con­junc­tion ­with flu­o­res­cence detec­tion ­allows ­high ­speed, quan­ti­ta­tive ­data gath­er­ing. Such micro­ar­ray ­assays ­have ­been pri­mar­i­ly ­applied to the mon­i­tor­ing of ­gene expres­sion, muta­tion detec­tion and ­many oth­er ­aspects of ­genome anal­y­sis. In ­this ­work, we ­report the use of a micro­ar­ray ­assay for the simul­ta­ne­ous, quan­ti­ta­tive deter­mi­na­tion of anti­bod­ies to Toxoplasma gon­dii, Rubella ­virus, Cytomegal­o­vi­rus and Herpes sim­plex ­virus ­types 1 and 2 (ToRCH pan­el). Microarrays of ­human IgG, ­human IgM and ­viral anti­gens ­were print­ed on ­glass ­slides ­using ­high ­speed robot­ics. After incu­ba­tion ­with sam­ples and flu­o­res­cent ­probes the ­slides ­were ­scanned ­using con­fo­cal micros­co­py and quan­ti­fied ­using the anti­body dilu­tion ­curves print­ed on the ­slides. Excellent agree­ment was ­obtained ­with ­enzyme immu­noas­say ­data. The ­test for­mat can be incor­po­rat­ed in a ful­ly auto­mat­ed ran­dom ­access immu­noas­say plat­form for rou­tine use in clin­i­cal chem­is­try labor­a­to­ries.

language: English


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