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Minerva Biotecnologica 2000 December;12(4):309-17

language: English

Encap­su­la­tion of ­Sertoli’s ­cells for in ­vivo trans­plan­ta­tion

Luca G. 1, Calafiore R. 2, Basta G. 2, Ricci M. 1, Rossi C. 1, Nastruzzi C. 1

1 Depart­ment of Medic­i­nal Chem­is­try and Phar­ma­ceu­tics, School of Med­i­cine, Uni­ver­sity of Peru­gia, Peru­gia, Ita­ly;
2 Depart­ment Inter­nal Med­i­cine, Sec­tion of Inter­nal Med­i­cine and Endo­crine and Meta­bol­ic Sci­enc­es, ­School of Med­i­cine, Uni­ver­sity of Peru­gia, Peru­gia, Ita­ly


The ­present ­paper ­describes the ­most ­recent advanc­es in the encap­su­la­tion of ­Sertoli’s ­cells (SC) for in ­vivo trans­plan­ta­tion. ­Recent ­reports ­have dem­on­strat­ed ­that ­Sertoli’s ­cells may ­improve ­both in ­vitro and in ­vivo func­tion­al com­pe­tence of dif­fer­ent ­cells; in particular, SC improve, in vitro, the functional competence of pancreatic islats (I) and, in ­vivo, pro­long ­islet allo­graft sur­vi­val in ani­mal mod­el of dia­betes mel­lit­us. We ­have devel­oped (a) a meth­od for ­high ­yield sep­ar­a­tion of pur­i­fied SC ­from ­rodents and por­cine pre-puber­tal ­testes and (b) dif­fer­ent meth­ods for the encap­su­la­tion of SC in algi­nate micro­cap­sules, name­ly, “Medi­um ­Size” (MSM) and “Coher­ent” Micro­cap­sules (CM). ­Both pro­ce­dures are rel­a­tive­ly sim­ple and con­sist­e of a lim­it­ed num­ber of ­steps. In ­order to ­achieve com­plete bio­com­pat­ibil­ity, indis­pens­able for mam­mal­ian ­cells, the encap­su­la­tion pro­ce­dures ­were con­duct­ed at ­room tem­per­a­ture ­under phys­io­log­ic pH and tonic­ity.

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