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A Journal on Biotechnology and Molecular Biology
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Minerva Biotecnologica 2000 December;12(4):271-8
The effect of different in vitro solutions in the dissolution of calcium pectinate beads intended for the delivery of live cells to the human large intestine
Chan W. A., Boswell C. D., Zhang Z.
School of Chemical Engineering, University of Birmingham, Edgbaston, B15 2TT, United Kingdom
Hydrogel formed by a low methoxyl pectin and calcium ion has potential application for targeted delivery of biological materials to the human large intestine. Pectin does not dissolve in the upper human gastrointestinal (GI) tract but can be degraded by pectinolytic enzymes present in the human large intestine. However, the composition of different types of in-vitro dissolution fluids designed to mimic human small intestine and large intestine fluids, can affect the dissolution profile of calcium pectinate (CaP) beads. The aim of this study was to compare the release profiles of a biological material in some commonly used phosphate buffer solutions with release profiles in physiological salts solutions, using the BP 98 paddle dissolution method. It was found that the release profiles for these two types of in-vitro solutions were comparable without the pre-treatment with an acid solution, but were markedly different following the acid treatment. In addition, it was found that the presence of calcium ion in the solution affected the activity of the pectinolytic enzyme.