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A Journal on Biotechnology and Molecular Biology

Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246

Frequency: Quarterly

ISSN 1120-4826

Online ISSN 1827-160X


Minerva Biotecnologica 2000 March;12(1):57-61


Mitochondria and apop­to­sis: func­tion­al stud­ies on mem­brane poten­tial (∆Ψ)

Cossarizza A.

Department of Biomedical Sciences, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy

Apoptosis is a phe­nom­e­non of cru­cial impor­tance in all mul­ti­cel­lu­lar, liv­ing organ­isms: it is ­involved in sev­er­al phys­io­log­i­cal and path­o­log­i­cal phe­nom­e­na ­which ­incluse embryo­gen­e­sis, dif­fe­ren­ti­a­tion, devel­op­ment of the ­immune ­system, as ­well as con­trol of ­cell ­growth, can­cer­o­gen­e­sis, ­viral pro­duc­tion. The assump­tion ­that apop­to­sis is an ­active pro­cess led to ­study mito­chon­dria, the ­main intra­cel­lu­lar ­source of ener­gy, ­which is pro­duced in ­form of ATP. It is rea­son­able to hypo­the­size ­that, at ­least in ear­ly apop­tot­ic phas­es, ­cells ­have to main­tain a ­good driv­ing ­force for ATP syn­the­sis, and, con­se­quent­ly, a ­high mit­o­chon­dri­al mem­brane poten­tial (∆Ψ), ­that ­becomes a reli­able mir­ror of the capac­ity to syn­the­size ATP and, in gen­er­al, of mit­o­chon­dri­al mem­brane func­tion­al­ity. In ­this arti­cle, the use of dif­fer­ent flu­o­res­cent ­probes for the anal­y­sis of ∆Ψ is dis­cussed pay­ing a par­tic­u­lar atten­tion for JC-1, ­that is con­sid­ered the gold­en stan­dard for ­this ­type of meas­ure.

language: English


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