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Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Online ISSN 1827-160X
FLOW CYTOMETRY IN IMMUNOLOGY AND HEMATOLOGY
Flow Cytometry Unit, Laboratory of Clinical Pathology, Venice General Hospital, Venice, Italy
Flow cytometry has been deeply modified by a continuous series of advances regarding the most diverse fields of modern technology. The most important improvement consists in its capacity to investigate several different parameters in the same analytical run, and then reprocess the data in a multivariate analysis. This important function has been allowed not only by the availability of multichannel flow cytometers, but also by the synthesis of fluorescent dyes with conveniently matched spectra. Although very popular, nevertheless a multiparametric approach is not a trivial task, inasmuch it relies on the firm control of each analytical variable and presupposes the fixing of the artefacts which can interfere with the measures. In this article some pivotal arguments are discussed, i.e. the choice of fluorochromes, the control of artefacts, and the analysis of data. Special emphasis has been put on the more tricky situations, like spectral and sterical incompatibility between dyes, and the spill-over between different channels.