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A Journal on Anesthesiology, Resuscitation, Analgesia and Intensive Care

Official Journal of the Italian Society of Anesthesiology, Analgesia, Resuscitation and Intensive Care
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Minerva Anestesiologica 2002 January-February;68(1-2):13-6

language: English

Antioxidant property of Propofol in the ischemic and reperfused human skeletal muscle

Corbucci G. G., Marchi A., Velluti C. *, Chelo C. *, Grella E. **, Lettieri B. **

From the Insti­tute of Anaesthe­sia and Inten­sive ­Care
*Insti­tute of Ortho­pe­dic and Trau­ma­tol­o­gy University of Cagliari
**Depart­ment of Anaesthe­sia and Inten­sive ­Care, II Uni­ver­sity of Nea­ples


Back­ground. Oxy­gen-­derived ­free rad­i­cals (ROS) are ­involved in tis­sue dam­age dur­ing mus­cle ische­mia and reper­fu­sion. ­Recent in ­vitro stud­ies ­have dem­on­strat­ed ­that a ben­e­fi­cial ­effect of Pro­pof­ol (2,6 dii­sop­ro­pyl­phe­nol) ­lies on its ­free rad­i­cal scav­eng­ing prop­er­ties. The cur­rent ­study there­fore exam­ined wheth­er Pro­pof­ol is effec­tive ­against the per­ox­i­da­tive dam­age ­induced by ROS in ­human skel­e­tal mus­cle in the ­course of ­acute ische­mia and reper­fu­sion.
Meth­ods. A homo­ge­ne­ous ­group of ­patients (n=20) under­go­ing ortho­pe­dic sur­gery was sub­ject­ed to hand­line tour­ni­quet at 350 At for 60 min fol­low­ing by 20 min post­is­chem­ic reper­fu­sion. In skel­e­tal mus­cle sam­ples (m. vas­tus lat.) mal­on­dyal­deide (MDA), cat­a­lase (CAT) and ­uric ­acid lev­els ­were ana­lyzed ­before tour­ni­quet appli­ca­tion, ­after 60 min of ische­mia and ­then ­after 20 min fol­low­ing reper­fu­sion. To ten sub­jects Pro­pof­ol was sup­plied as ­bolus (5 mg/kg, ­body ­weight) dur­ing the ischem­ic inter­val. The tis­sue con­cen­tra­tions of MDA, CAT and ­Uric ­Acid ­were meas­ured by spec­tro­pho­to­met­ric and phlu­or­i­met­ric meth­ods com­par­ing the val­ues ­with the ­data ­obtained in an untreat­ed ­group of ­patients (n=10).
­Results. In all ­patients ischem­ic inju­ry sig­nif­i­cant­ly ­increased MDA, and ­Uric ­Acid con­tents ­with a con­com­i­tant ­decrease in CAT lev­els. ­When reper­fused the Pro­pof­ol treat­ed ­group ­showed an evi­dent ­decrease in MDA ­Uric, and CAT gra­dients in ­respect of ischem­ic tis­sue. On the con­trary rap­id reoxy­gen­a­tion ­implies a high­ly sig­nif­i­cant ­increase in MDA as far as ­Uric ­Acid con­tents, ­while Cat­a­lase lev­els ­were ­unchanged.
Con­clu­sions. The cur­rent ­study dem­on­strat­ed ­that in the ­human skel­e­tal mus­cle Pro­pof­ol atten­u­ates the lip­id per­ox­i­da­tion ­induced by ische­mia and reper­fu­sion and ­this ben­e­fi­cial ­action of Pro­pof­ol is prob­ably cor­re­lat­ed ­with the ­free rad­i­cal scav­eng­ing prop­er­ties of ­this mole­cule.

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