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A Journal on Anesthesiology, Resuscitation, Analgesia and Intensive Care
ORIGINAL ARTICLES CRITICAL AND INTENSIVE THERAPY
Minerva Anestesiologica 2001 May;67(5):387-92
The nitric oxide metabolism in the hypoxic, ischemic and reperfused human skeletal muscle cell: clinical and therapeutical observation
Corbucci G. G., Palmerini C. *, Palombari R. **, Lettieri B., Grella E., Velluti C. ***, Chelo C. ***
Università degli Studi - Cagliari
Istituto di Anestesia-Rianimazione
°II Università degli Studi - Napoli
Dipartimento di Anestesia-Rianimazione,
*Università degli Studi - Perugia Dipartimento Biologia Cellulare e Molecolare
**Dipartimento di Chimica
*** Università degli Studi - Cagliari Dipartimento di Ortopedia e Traumatologia
Background. The biochemical and metabolic role played by nitric oxide (NO) in course of oxidative stress due to cell hypoxia, ischemia and reperfusion has a determinant relevance in the mitochondrial adaptive changes which antagonize the irreversible morpho-functional damage. In particular conditions, such as in prolonged ischemia and/or exogenous NO supplementation, this element is present in the radicalic form (·NOO) concurring to peroxidative cell injury. Aim of this study was to investigate these opposite NO aspects in hypoxic, ischemic and reperfused human skeletal muscle tissue.
Methods. Skeletal muscle samples were taken during elective knee orthopedic surgery in 10 consecutive patients. The biopsies were obtained before, after 5±1 min and 58±2 min from tourniquet application and then after 18±3 min following muscle reperfusion. The samples, immediately frozen in liquid nitrogen, were assayed for endocellular free NO following the gas-amperometric method described by Palmerini C.
Results. When compared with normoxic tissues, a significant decrease in free NO content was observed in hypoxic samples. After about 60 min of prolonged ischemia the NO levels show an evident increase, while the tissue reperfusion leads to a progressive restoration of physiological content in the cellular free nitric oxide.
Conclusions. The obtained data in hypoxic muscle cell seem to underline the pivotal role played by NO in adapting the cytochrome c oxidase oxidative activity to lower O2 bio-availability. On the other hand the prolonged ischemia leads to a consistent ·NOO generation triggered by oxyradical generation and Ca2+ intracellular over load. Even if the tissue reoxygenation restores the normal NO levels it is arguable that the pre-treatment of ischemic cell with antioxidants, Ca2-antagonist and Desamethasone supplementation could represent a crucial and specific therapeutic approach to critically ill patient.