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Official Journal of the , the International Union of Phlebology and the
Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,899
Online ISSN 1827-1839
Wu Y. F. 1, He F. L. 2, Gu Y. Q. 1, Chen X. S. 3, Chen L. 3, Chen L. 4, Zhang J. 1, Wang Z. G. 1
1 Deparment of Vascular Surgery, Xuan Wu Hospital and Institute of Vascular Surgery, Capital Medical University, Beijing, China;
2 Interventional Therapy Department, Shijitan Hospital, Capital Medical, Beijing, China;
3 Deparment of Urology, Xuan Wu Hospital, Capital Medical University, Beijing, China;
4 National institute for the Control of Pharmaceutical and Biological Products, Beijing, China
AIM: Aim of the study was to construct and evaluate of autologous cell derived vein grafts based on tissue engineering concept.
METHODS: In this study, we constructed venous grafts (VGs) in 12 days based on tissue engineering concept. We draw out 8-12 mL of bone marrow from the intended recipient canines (N.=8) to culture and expand endothelial progenitor cells (EPCs). After having been labeled with PKH26-GL, the cells were seeded onto the luminal surface of decellularized scaffolds (DSs) with single, rotative method for 4 hours. Following static culture for 24-72 hours, the hybrids were implanted to recipient canine inferior vena cava. Non-seeded DSs (N.=4) were performed as control.
RESULTS: Angiography disclosed that patent number of test (control) group were 7/7 (2/4), 6/6 (2/2) and 4/4 (1/2) at postoperative 10 days, 4 weeks and 12 weeks, respectively. At 12 weeks, confluenced endothelial cells which covered the whole inner luminal surface of the explants were detected. Meanwhile, fibroblasts and α-actin positive cells in the matrices were found. PKH26-GL labeled EPCs sustained on the luminal surface accompanied by newly formed endothelial cells. However, the explants in both groups showed partial stenosis.
CONCLUSION: These results indicate that such constructed VGs based on autologous bone marrow-derived EPCs and porcine DSs are promising and deserve to further improvement and testing.