Advanced Search

Home > Journals > International Angiology > Past Issues > International Angiology 2008 December;27(6) > International Angiology 2008 December;27(6):529-35

ISSUES AND ARTICLES   MOST READ   eTOC

CURRENT ISSUEINTERNATIONAL ANGIOLOGY

A Journal on Angiology

Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,899

Frequency: Bi-Monthly

ISSN 0392-9590

Online ISSN 1827-1839

 

International Angiology 2008 December;27(6):529-35

    Original articles

Glycosaminoglycan-degrading enzymes in the varicose vein wall

Kowalewski R. 1, Sobolewski K. 2, Malkowski A. 2, Gacko M. 1, Rutkowska I. 3

1 Department of Vascular Surgery and Transplantology, Medical University of Bialystok, Bialystok, Poland
2 Department of Medical Biochemistry, Medical University of Bialystok, Bialystok, Poland
3 Department of Rehabilitation, Medical University of Bialystok, Bialystok, Poland

Aim. Mechanical properties of the vein wall are determined by extracellular matrix components, including glycosaminoglycans (GAGs). The aim of the study was to evaluate the activity of enzymes involved in GAGs degradation pathway in the wall of varicose veins and varicose veins complicated by thrombophlebitis, when compared to the wall of normal ones.
Methods. Normal, varicose veins and varicose veins complicated by thrombophlebitis were collected during surgical treatment of 10 patients. Activities of endoglycosidases, sulphatases and exoglycosidases were assessed according to colorimetric methods.
Results. Activities of neutral endoglycosidases degrading chondroitin-4-sulphate (C4S) and heparan sulphate (HS) were decreased, whereas activities of neutral endoglycosidases degrading dermatan sulphate and hyaluronic acid were increased in varicose veins and varicose veins complicated by thrombophlebitis. Activities of acidic endoglycosidases degrading C4S and HS were decreased in varicose veins and varicose veins complicated by thrombophlebitis, whereas activity of acidic endoglycosidases degrading chondroitin-6-sulphate was decreased only in varicose veins complicated by thrombophlebitis. Furthermore increased activities of arylosulphatase B, β-N-acetylhexosaminidase and α-L-iduronidase were demonstrated in varicose veins, as well as in varicose veins complicated by thrombophlebitis.
Conclusion. Changed activities of GAGs-degrading enzymes may contribute to previously reported changes in the content and molecular differentiation of GAGs in the wall of varicose veins that may play a role in the disease pathogenesis.

language: English


FULL TEXT  REPRINTS

top of page