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INTERNATIONAL ANGIOLOGY

A Journal on Angiology


Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
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International Angiology 1999 December;18(4):331-4

Copyright © 2000 EDIZIONI MINERVA MEDICA

language: English

Effect of glycated low density lipoprotein on smooth muscle cell proliferation

Makita T., Tanaka A., Numano F.

From the Third Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo, Japan


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Back­ground. We inves­ti­gat­ed the ­effect of gly­cat­ed low den­sity lip­o­pro­tein (LDL) on smooth mus­cle cell pro­life­ra­tion.
Meth­ods. Blood was drawn from 6 ­healthy sub­jects after over­night fast­ing. ­Native LDL was ­obtained by sep­ar­at­ing LDL from the sam­ples with sequen­tial ultra­cen­trif­u­ga­tion. Gly­cat­ed LDL was pre­pared by gly­cat­ing the ­native LDL in vitro. ­Native and gly­cat­ed LDL were added to a medi­um con­tain­ing cul­tured por­cine cor­o­nary ­artery ­smooth mus­cle cells, and the ­change in cell pro­life­ra­tion was exam­ined after 24, 48, 72, and 96 hs. The cells were count­ed using a cell count­ing kit (Dojin Chem­i­cal Co., Ltd.).
­Results. There was no sig­nif­i­cant dif­fer­ence in the cell count ­between the con­trol group, in which only PBS was added, and the ­native LDL group. How­ev­er, cell pro­life­ra­tion was appre­ciably high­er in the gly­cat­ed LDL group than in the ­native LDL group. The mean total cell count at 24, 48, 72 and 96 hs was sig­nif­i­cant­ly high­er (p<0.01) in the gly­cat­ed LDL group (­median: 0.843; range: 0.576-1.060) than in the ­native LDL group (­median: 0.541; range: 0.282-0.683).
Con­clu­sions. These find­ings sug­gest that gly­cat­ed LDL induc­es sig­nif­i­cant­ly great­er accel­er­a­tion of ­smooth mus­cle cell pro­life­ra­tion than does ­native LDL. There­fore, the accel­er­a­tion of ­smooth mus­cle cell pro­life­ra­tion ­requires mod­ifi­ca­tion of LDL.

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