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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE
A Journal on Internal Medicine and Pharmacology
Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index
Gazzetta Medica Italiana Archivio per le Scienze Mediche 2016 January-February;175(1-2):15-22
The effects of cold stimulations of various temperatures on cell growth and metabolic activity of human cells in vitro
Takehito SUGASAWA 1, Naoki MUKAI 2, Satoru OSHIRO 3, Takafumi HANDA 4, Ikumi TANABE 3, Yuko KUMAKI 4, Kyoko TAMURA 4, Taiko TANBA 4, Norihiko MORIYAMA 5, Kazuhiro TAKEKOSHI 6
1 Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan; 2 Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan; 3 Division of Cell Biology, Department of Health Science, Graduate School of Sports and Health Science, Daito Bunka University, Higashi-Matsuyama, Saitama, Japan; 4 Department of Health Science, Faculty of Sports and Health Science, Daito Bunka University, Higashi-matsuyama, Saitama, Japan; 5 Majors of Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan; 6 Division of Sports Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan
BACKGROUND: The present study aimed to clarify the effects of cold stimulations of various temperatures on metabolic activity and cell growth in human cells in vitro.
METHODS: Two rhabdomyosarcoma (SJCRH30) and liposarcoma (SW872) cell lines were used in this study. For each cell type, the cells were divided into four groups and incubated in various temperatures (0, 4, 17, and 25°C) three times for 15 min each. The control cells were cultured at 37°C throughout the experiment. After the last cold stimulation, the metabolic activity and cell growth were analyzed by Alamar Blue and cell growth assays, respectively.
RESULTS: For SJCRH30, the Δ reducing rate, an indicator of metabolic activity, in the Alamar Blue assay was significantly higher in the 17°C cells compared with all other cells 7 hours after the assay was started. For SW872, the Δ reducing rate was significantly higher in the 25°C cells compared with all other cells 4 hours after the assay was started. For SJCRH30, the relative cell number in the cell growth assay was significantly higher in the 17°C cells compared with all other cells; whereas for SW872, the relative cell number was significantly higher in the 25°C cells compared with the control, 0°C, and 4°C cells.
CONCLUSIONS: Taken together, our data indicate that cold stimulation at specific temperatures for each cell type results in increased metabolism and cell growth. Hence, different applied cold temperatures for injured areas may result in different therapeutic effects, indicating that the management of cold temperature is indeed important for sports injuries.