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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE
A Journal on Internal Medicine and Pharmacology
Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index
Gazzetta Medica Italiana Archivio per le Scienze Mediche 2015 October;174(10):431-9
Role of a new acid-oxidant solution in the control of wounds microenvironment
D’Atanasio N. 1, Capezzone De Joannon A. 1, Mangano G. 1, Giarratana N. 2, Milanese C. 1, Tongiani S. 1
1 Angelini S.p.A. - R & D, Angelini Research Center S.Palomba, Pomezia, Roma, Italia;
2 APR Applied Pharma Research SA, Balerna, Svizzera
AIM: The role in modulating the wound microenvironment of a novel acidOxidizing solution containing free chlorine, >95% as hypochlorous acid (Amuphase, Nexodyn Acid Oxidizing Solution AOSTM), a class III medical device, has been assessed. The product has also been tested for compatibility and absence of irritating action on the skin as well as for its impact on the process of wound healing.
METHODS: The antibacterial and antiviral activity has been evaluated in vitro according to standard procedures; the tolerability of the product was analyzed both in vitro, with a cytotoxicity test on a murine cell line, and in vivo on rabbit skin after single or repeated application for 4 weeks; finally, the effects on wound closure were assessed in vivo in the porcine full-thickness excisional wound model after application of Amuphase for 10 days.
RESULTS AND CONCLUSION: 1) the product exerts antimicrobial activity on the examined strains of bacteria and viruses and shows no cytotoxic effects after 24 hours in vitro nor irritating activity in vivo after single or repeated application; 2) the in vivo study on the excisional wound model showed that Amuphase creates a microenvironment that influences the recruitment of neutrophils at the site of the lesion favoring the dynamics leading to the physiological healing of wounds. Furthermore, 3) in in vitro studies on metalloproteases it has been observed that the physicochemical characteristics of Amuphase enable it to inhibit the proteolytic activity of MMP-2 and MMP-9, factors that interfere negatively with the wound healing process.