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Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index
Online ISSN 1827-1812
Yushkov B. 1, 2, Tyumentseva N. 2, Khodakov V. 3, Medvedeva S. 1, 2, Krokhin D. 2, Plaksin K. 3, Rantsev M. 2, 3, Sarapultsev P. 1, 2, Sarapultsev A. 1, 2
1 Federal State Autonomous, Educational Institution of Higher Professional Education, Ural Federal University Named After, the First President of Russia B. N. Yeltsin Ekaterinburg, Russia;
2 Institute of Immunology and Physiology of the Ural Division of Russian Academy of Sciences, Ekaterinburg, Russia;
3 Department of General Surgery, Ural State Medical University, Ekaterinburg, Russia
AIM: The objective of this research was to develop the technique of receiving connective-tissue autoprostheses and to assess their performance characteristics after they have been auto-transplanted into arterial vessels.
METHODS: The 5-week old connective-tissue prostheses were used for plastic repair of carotid arteries and femoral arteries of rats and rabbits, respectively, which served as the source body for building these prostheses. The prostheses were sewn end-to-end into the transected vessel. The ultrasonic and angiographic examinations showed that the prostheses remained patent and manifested normal functions 3 and 6 months after the surgery. No signs of thrombosis or occlusion were detected, which is confirmed by the histological analyses. The electronic microscopy indicated that endothelial cells started to develop on the inner surface of the autoprostheses.
RESULTS: The experiments performed on rats and rabbits have shown the possibility of development of a connective-tissue prosthesis on the PVC scaffold inserted under the skin. The development goes through gradual thinning of the wall, formation of fibrous connective tissue and arrangement of collagen fibers, reduction in the number of cells per unit area and predominance of more mature fibrocyte cells.
CONCLUSION: The performed experimental research shows that connective-tissue prostheses can be built in animals both with the underdeveloped and with the well-defined subcutaneous fat layer. The autoprostheses received under the proposed technique feature biological inertia and good plastic characteristics; they are thrombosis-resistant and sufficiently strong.