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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE

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Gazzetta Medica Italiana Archivio per le Scienze Mediche 2013 December;172(12):893-900

language: English

Culture of adult human bone marrow stem cells on fresh-frozen allograft bone

Drumond Loro R. C. 1, Viezzer C. 2, Wehmeyer Fregapani P. 3, Gerhardt De Oliveira M. 1, Cantarelli Machado D. 4

1 Oral and Maxillofacial Surgery Department Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil;
2 Mining, Steel, and Materials Engineering Universidade Federal do Rio Grande do Sul Porto Alegre, Brazil;
3 Oral and Maxillofacial Surgery Program Universidade Luterana do Brasil Canoas, RS, Brazil;
4 School of Medicine, Biomedical Research Institute Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil


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Aim: The objective of this study was to assess the ability of fresh-frozen allograft bone and of diagnostic anaerobic growth medium to promote osteogenic differentiation of adult human bone marrow stem cells in vitro.
Methods: Morphological characteristics of osteogenic cells and of the allograft and the expression of bone proteins osteopontin, osteocalcin, and alkaline phosphatase were analyzed at 7, 14, and 21 days of culture with and without diagnostic anaerobic growth osteogenic medium.
Results: The comparison of results obtained at 7 days and at 21 days revealed an increase in cell adhesion, migration, growth, and differentiation, in addition to bone mineralization, in all cultures. Diagnostic anaerobic growth accelerated the osteogenic process, especially in the first two weeks.
Conclusion: Fresh-frozen allograft showed adequate osteoinductive and osteoconductive properties when associated with adult stem cells and proved to be an adequate scaffold for osteogenesis.

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