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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE
A Journal on Internal Medicine and Pharmacology
Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index
Gazzetta Medica Italiana Archivio per le Scienze Mediche 2010 October;169(5):157-61
Serum total sialic acid, lipid peroxidation, ceruloplasmin and glutathione reductase levels in patients with osteoarthritis
Surapaneni Krishna M., Vishnu Priya V.
Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha University, Chennai, Tamilnadu, India
Aim. The changes in the serum Sialic acid levels, Total lipid peroxidation products (MDA), Ceruloplasmin (a non enzymatic antioxidant) and Glutathione Reductase activity were estimated in patients with osteoarthritis. Serum Sialic acid is known as a parameter of inflammation. This work was undertaken to assess the potential role of Sialic acid as well as oxidative stress and anti oxidant status in patients with osteoarthritis.
Methods. The study was conducted in thirty patients and compared to controls. Levels of serum total sialic acid, MDA, ceruloplasmin and activity of antioxidant enzyme – glutathione reductase were measured in patients with osteoarthritis. Serum total sialic acid levels were determined by using thiobarbituric acid assay method described by warren. Serum MDA was determined as the measure of thio barbituric acid reactive substances (TBARS). Ceruloplasmin, a non enzymatic antioxidant levels was measured by the method of R avin and Glutathione reductase activity was measured in the serum by the method described by Goldberg DM. These parameters were measured in thirty patients and compared to controls. Statistical analysis between group 1 (controls) and group 2 (patients) was performed by the Mann Whitney U test. The data were expressed as mean + SD. P < 0.05 was considered as significant.
Results. It was observed that there was a significant increase in serum Sialic acid, MDA levels and glutathione Reductase activity along with the decrease in ceruloplasmin levels in patients with osteoarthritis when compared to controls.
Conclusion. The results of our study suggest higher oxygen free radical production, evidenced by increased MDA and decreased ceruloplasmin levels support to the oxidative stress in osteoarthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response to increased oxidative stress. The results suggest the antioxidant activity of Sialic acid in response to the increased oxidative stress in osteoarthritis.